Fig 1: SC-PDCC clones capture intratumoral heterogeneity of drug response. A) Schematic procedure of establishing SC-PDCC clones from CRC patient. Colonies were generated after processing tissues (PDCC P0). Scale bar: 50 µm. Then cells were sorted using EPCAM+ to obtain single cell per well. Scale bar: 100 µm. SC-PDCC clones were established after expansion. Scale bar: 50 µm. B) Representative bright field microscopy images of SC-PDCC colonies and H&E images of SC-PDCCs derived ALI organotypic cultures. Passage numbers were marked in brackets. Scale bar: 50 µm. C) Rhodamine staining showing that all SC-PDCC clones of P62 are resistant to 10 × 10-6 m PLX4720, but their responses to gefitinib are different. All PLX4720 resistant SC-PDCC clones are sensitive to the combination of 10 × 10-6 m PLX4720 and 10 × 10-6 m gefitinib. Three technical replicates are performed for each clone. D) PCA showed that SC-PDCC clones with different response to gefitinib are separated into two groups. E) GSEA showing the significantly enriched EGF response pathway in gefitinib sensitive SC-PDCC clones.